Highlights:

• Affinitymatrixforstablecarbodiimide-basedcouplingofligandscontainingaminogroups.
• Ligandsarecoupledinasingle-stepprocedureinthepresenceofacouplingreagent(notprovided)whichisawater-solublecarbodiimide,suchasN-ethyl-N`-(3-dimethylaminopropyl)carbodiimidehydrochloride(EDC).
• Thematrixprovidesa10-atomspacerarmconvenientforcouplingofsmallligands.
• Noblockingreactionisnecessaryfollowingthecouplingreaction.
• IdealforimmobilizationofsmallligandsstableinalowpHandcontainingprimaryamines.
• Couplingreactioncanbecarriedoutinorganicsolvent.

Technicalspecifications:

• Matrixactivegroup:-COOHgroups
• Matrix:4B(agarosebeads,4%)
• Particlesizerange:52–165μm
• Molecularweightrange:6x10⁴ -2x10⁷
• Grouptobecoupled:-NH₂ groups
• Chemicallinkageofligand:Stableamidelinkage
• Liganddensity:12–16μmolcarboxylgroups/mldrainedmatrix
• Spacerarm:6-aminocaproicacid,10atoms
• CouplingconditionspH4.5–6,Temp:4°C–25°C,Time:1.5–24h;organicsolventspossIBLe
• Storage:2–8°C

AdditionalInformation

AdditionalInformation