DTT;Cleland"sReagent

C-1029

3483-12-3

(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol

C₄H₁₀O₂S₂

154.25Da

Whitecrystallinepowder

≥99.0%

10%solutioninwater.Clearandcolorless

42-43°C

N/A

+4°C,underinertgas,desiccated

NotApplicable

DTTisfrequentlyusedtoreducethedisulfidebondsofproteinsand,moregenerally,topreventintramolecularandintermoleculardisulfidebondsfromformingbetweencysteineresiduesofproteins.However,evenDTTcannotreduceburied(solvent-inaccessIBLe)disulfidebonds,soreductionofdisulfidebondsissometimescarriedoutunderdenaturingconditions(e.g.,athightemperatures,orinthepresenceofastrongdenaturantsuchas6Mguanidiniumchloride,8Murea,or1%sodiumdodecylsulfate).DTTisoftentimesusedalongwithsodiumdodecylsulfateinSDS-PAGEtofurtherdenatureproteinsbyreducingtheirdisulfidebondstoallowforbetterseparationofproteinsduringelectrophoresis.BecauseoftheABIlitytoreducedisulfidebonds,DTTcanbeusedtodenatureCD38onredbloodcells.Conversely,thesolventexposureofdifferentdisulfidebondscanbeassayedbytheirrateofreductioninthepresenceofDTT.DTTcanalsobeusedasanoxidizingagent.Itsprincipaladvantageisthateffectivelynomixed-disulfidespeciesarepopulated,incontrasttootheragentssuchasglutathione.Inveryrarecases,aDTTadductmaybeformed,i.e.,thetwosulfuratomsofDTTmayformdisulfidebondstodifferentsulfuratoms;insuchcases,DTTcannotcyclizesinceithasnosuchremainingfreethiols.

12.3441.1996

MFCD00004877

X1009950-2

InChI=1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

C([C@H]([C@@H](CS)O)O)S

EK1610000

UN3335

III

Hygroscopic.Protectfrommoisture.Protectfromlight.Thepharmacologicalandtoxicologicalpropertiesofthisproducthavenotbeenfullyinvestigated.Usecautionwhenhandling.Donotusethiscompoundifyouarenotfullytrainedorareunawareofthehazardsinvolved.

C-1029,DTT,SDS,diamondformat.pdf

C-1029,J1220.pdf

C-1029,H1422B.pdf

C-1029,H1422A.pdf