More Information
Alternate Name/Synonyms
Blasticidin S; Bla-S
Chemical Name
(2S,3S,6R)-3-[[(3S)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride
Chemical Formula
C17H26N8O5•HCl
Solubility
Soluble in water. Clear and faint yellow solution at 5 mg/ml in water. Insoluble in organic solvents.
Melting Point
218.0-225.0 °C
Storage Temp
+4°C, desiccated
THERAPEUTIC AREA
Antibacterial
USE
Blasticidin is an efficient selection antibiotic that acts on both eukaryotic and prokaryotic cells. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery. In biological research, specifically genetic engineering, it is used to select transformed cells which have been engineered to carry a resistance gene for blasticidin. In short, DNA of interest is fused to DNA encoding a resistance gene, and then is transformed into cells. After allowing time for recovery and for cells to begin transcribing and translating their new DNA, blasticidin is added. Now only the cells that have the new DNA can grow. Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase. Resistance to blasticidin is also conferred by the blastcidin S acetyltransferase gene (bls) from Streptoverticillum sp, and the blasticidin S deaminase gene (BSD) from Apergillus terreus. Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week.
INCHI
InChI=1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H
SMILES
[H]/N=C(N)/N(C)CCC(CC(=O)NC1C=CC(OC1C(=O)O)n2ccc(nc2=O)N)N.Cl
Peptide sequence
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Avoid contact with skin, eyes or clothing. Use personal protective equipment as required.Wash contaminated clothing before reuse. Do not breathe dust/fume/gas/mist/vapors/spray.Do not eat, drink or smoke when using this product. Corrosive hazard. Wear protectivegloves/clothing and eye/face protection. Thermal decomposition can lead to release oftoxic/corrosive gases and vapors.
Citations
VIRUS-CELL INTERACTIONS:Human Apolipoprotein E Is Required for Infectivity and Production of Hepatitis C Virus in Cell CultureKyung-Soo Chang, Jieyun Jiang, Zhaohui Cai, and Guangxiang LuoJ. Virol., Dec 2007; 81: 13783 - 13793.
Citations_Hyperlink
http://jvi.asm.org/cgi/reprint/81/24/13783
msds 1
B-1247, Blasticidin S HCl, SDS, diamond format.pdf
Certificate of Analysis 1
B-1247, J1268.pdf
Certificate of Analysis 2
B-1247, H1272.pdf
Certificate of Analysis 3
B-1247, H1252A.pdf
AG Scientific常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AG Scientific/Proteinase K/500 mg/199.99 | 199.99 | AG Scientific, Inc. | 蛋白酶类 | 0.00 |
AG Scientific/Proteinase K/50 mg/39.99 | 39.99 | AG Scientific, Inc. | 其他试剂 | 0.00 | AG Scientific/Proteinase K/100 mg/59.99 | 59.99 | AG Scientific, Inc. | 单克隆抗体 | 0.00 | AG Scientific/Proteinase K (Recombinant), Molecular/PCR Grade/1 g/537.62 | 537.62 | AG Scientific, Inc. | 琼脂糖 | 0.00 | AG Scientific/Proteinase K Solution (20 mg/mL)/5 ml/123.29 | 123.29 | AG Scientific, Inc. | 刺激/抑制试剂 | 0.00 |