ProteaseK;EndopeptidaseK;Tritirachiumalkalineproteinase

P-1264

29.3kDa

Whitetooff-whitelyophilizedsolidwithnoadditives

≥99%

50mMTris-HCl(pH7.5),3mMCaCl2,50%Glycerol

4°C,desiccated

NotAvailable

ThisPCRGradeProteinaseKisparticularlysuitableforisolatingnucleicacidsforamplificationreactions,aswellasfortheanalysisofmembranestructuresbymodifyingproteinsandglycoproteinsoncellsurfaces.ItisextremelyeffectiveonnativeproteinsandcanthereforebeusedtorapidlyinactivateendogenousnucleasessuchasRNasesandDNasesduringnucleicacidisolation.ThispropertymakesProteinaseKparticularlysuitablefortheisolationofnativeRNAandDNAfromtissuesorcelllines.Itpromotescelllysisbyactivatingabacterialautolyticfactor.BecausetheenzymeistestedfortheabsenceofRNasesandDNases,andisvirtuallyfreeofDNA,itisespeciallysuitableforisolatingPCRandRT-PCRtemplates.ProteinaseKcanalsobeusedtoremovecellulardebrisduringthepreparationofcolonylifts,andtotreattissuesectionstoensureefficientprobeinfiltrationduringinsituhybridization.

Wearpersonalprotectiveequipment.Ensureadequateventilation.Donotgetineyes,onskin,oronclothing.Avoidingestionandinhalation.Avoiddustformation.Washhandsbeforebreaksandimmediatelyafterhandlingtheproduct.

P-1264,G1340A.pdf

P-1264,G1340.pdf