LeupeptinisareversIBLecompetitiveinhibitorofserineandthiolproteases.Ithasbeenreportedtoinhibitcalpain,cathepsinB,cathepsinsH,Landtrypsin.Typicalworkingconcentrationsareintherangeof10to100µM.Leupeptinappearstobeequallyeffectiveinanysaltform,adjustingforequivalentpeptidecontent.Thehemisulfatesaltswerethefirsttobecommerciallyavailable.Ofthethreesalts,thehydrochlorideistheleastinvasiveforminBIOLOGicalsettings.Noproblemorpreferenceforthetrifluoroacetate(TFA)formhasbeennoted;TFAisvolatile,socouldpossiblyberemovedbylyophilization.
Microbiallyproducedleupeptininhibitorwasfirstisolatedasamixtureoftwoverysimilarforms:acetyl-LeuLeu-Arg-alandpropionyl-Leu-Leu-Arg-al.Althoughthepropionylleupeptinisactiveasaninhibitortheacetylformismorecommonlyusedandisavailableinseveraldifferentforms. Leupeptin,becauseofitsaldehydegroup,mayactasareducingagentandthereforeinterfereinproteindeterminationssuchasLowryand,toalesserextent,Bradford.
Leupeptinstock(20mg/ml):LeupeptininMethanol.Prepareastockof20mg/mlinmethanol,Storeat-20°C.
Synonyms | Ac-Leu-Leu-Arginal | |||
---|---|---|---|---|
Product# | L-1165 | |||
CAS# | 103476-89-7 | |||
ChemicalName | Acetyl-Leu-Leu-Arg-al;Acetyl-L-leucyl-L-leucyl-L-argininal.hemisulfate | |||
Formula | C20H38N6O4.0.5H2SO4 | |||
MW | 475.6 | |||
Appearance | Whitetofaintyellowpowder | |||
Purity | >90%byHPLC,typically~>96.0% | |||
Solubility | SolubleinH20(50mg/ml).Clearcolorlesstofaintyellowsolution | |||
StorageTemp | -20°C |
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AG Scientific/Proteinase K/500 mg/199.99 | 199.99 | AG Scientific, Inc. | 蛋白酶类 | 0.00 | AG Scientific/Proteinase K/50 mg/39.99 | 39.99 | AG Scientific, Inc. | 其他试剂 | 0.00 | AG Scientific/Proteinase K/100 mg/59.99 | 59.99 | AG Scientific, Inc. | 单克隆抗体 | 0.00 | AG Scientific/Proteinase K (Recombinant), Molecular/PCR Grade/1 g/537.62 | 537.62 | AG Scientific, Inc. | 琼脂糖 | 0.00 | AG Scientific/Proteinase K Solution (20 mg/mL)/5 ml/123.29 | 123.29 | AG Scientific, Inc. | 刺激/抑制试剂 | 0.00 |